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CBM Technology – IT Services
Rapid detection of bacteria responsible for foodborne diseases is a growing necessity for public health. Reporter bacteriophages phages are robust biorecognition elements uniquely suited for the rapid and sensitive detection of bacterial species.
The advantages of phages include their host specificity, ability to distinguish viable and non-viable cells, low cost, and ease of genetic engineering. Upon infection with reporter phages, target bacteria express reporter enzymes encoded within the phage genome.
In this study, the T7 coliphage was genetically engineered to express the newly developed luceriferase, NanoLuc NLucas an indicator of bacterial contamination.
While several genetic approaches were employed to optimize reporter enzyme expression, the novel cbj of this work was the successful fusion of the NanoLuc reporter to a carbohydrate binding module CBM with specificity to crystalline cellulose.
This novel chimeric reporter nluc:: We have shown the possibility of detecting the immobilized fusion protein in a filter plate which resulted from a single CFU of E. Therefore, we conclude that our phage-based detection assay displays significant aptitude as a proof-of-concept drinking water diagnostic assay for the low-cost, rapid and sensitive detection of E. The article was received on 26 Apraccepted on 11 Jul and first published on 02 Cgm Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.
Information about reproducing material from RSC articles with dbm licences is available on our Permission Requests page.
50m Self-propelled Cutter Suction Dredger – CBM per hour – Horizon Ship Brokers, Inc.
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CBM fusion for the ultrasensitive detection of Escherichia coli in water. This article is part of the themed collections: Back to tab navigation Download options Please wait CBM fusion for the ultrasensitive detection of Escherichia coli in water T.
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